Wortmannin

Wortmannin, a metabolite of the fungi Penicillium funiculosum, [Talaromyces (Penicillium) wortmannii]] , is a specific inhibitor of phosphoinositide 3-kinases (PI3Ks). It has an in vitro inhibitory concentration (IC50) of around 5 nM, making it a more potent inhibitor than LY294002, another commonly used PI3K inhibitor. It displays a similar potency in vitro for the class I, II, and III PI3K members although it can also inhibit other PI3K-related enzymes such as mTOR, DNA-PK, some phosphatidylinositol 4-kinases, myosin light chain kinase (MLCK) and mitogen-activated protein kinase (MAPK) at high concentrations, Wortmannin has also been reported to inhibit members of the polo-like kinase family with IC50 in the same range as for PI3K. . The half-life of wortmannin in tissue culture is about 10 minutes. Wortmannin is a commonly used cell biology reagent that has been used previously in research to inhibit DNA repair, receptor-mediated endocytosis and cell proliferation.