Pseudomonas syringae

Pseudomonas syringae is a rod shaped, Gram-negative bacterium, with polar flagella. It is a member of the Pseudomonas genus, and based on 16S rRNA analysis, P. syringae has been placed in the P. syringae group. It is a plant pathogen which can infect a wide range of plant species, and exists as over 50 different pathovars. Many of these pathovars were once considered to be individual species within the Pseudomonas genus, but molecular biology techniques such as DNA hybridization have shown these to in fact all be part of the P. syringae species. It is named after the lilac tree (Syringa vulgaris), from which it was first isolated.

P. syringae tests negative for arginine dihydrolase and oxidase activity, and forms the polymer levan on sucrose nutrient agar. It is known to secrete the lipodepsinonapeptide plant toxin syringomycin, and it owes its yellow fluorescent appearance when cultured in vitro on King's B medium to production of the siderophore pyoverdin. It also demonstrates ice nucleating properties.

Epidemiology
Disease by P. syringae tends to be favoured by wet, cool conditions - optimum temperatures for disease tend to be around 12–25°C, although this can vary according to the pathovar involved. The bacteria tend to be seed borne, and are dispersed between plants via rain splash. .

Although it is a plant pathogen, it can also live as a saprophyte in the phyllosphere when conditions are not favourable for disease. Some saprophytic strains of P. syringae have been used as biocontrol agents against post-harvest rots.

Pathovars
Following ribotypical analysis several pathovars of Pseudomonas syringae were incorporated into other species (see P. amygdali, P. tomato, P. coronafaciens, P. avellanae, P. helianthi, P. tremae, P. cannabina, and P. viridiflava). The remaining pathovars are as follows:

Pseudomonas syringae pv. aceris attacks maple Acer species.

Pseudomonas syringae pv. aptata attacks beets Beta vulgaris.

Pseudomonas syringae pv. atrofaciens attacks wheat Triticum aestivum.

Pseudomonas syringae pv. dysoxylis attacks the kohekohe tree Dysoxylum spectabile.

Pseudomonas syringae pv. japonica attacks barley Hordeum vulgare.

Pseudomonas syringae pv. lapsa attacks wheat Triticum aestivum.

Pseudomonas syringae pv. panici attacks Panicum grass species.

Pseudomonas syringae pv. papulans attacks crabapple Malus sylvestris species.

Pseudomonas syringae pv. pisi attacks peas Pisum sativum.

Pseudomonas syringae pv. syringae attacks Syringa and Phaseolus species.

Note that Pseudomonas savastanoi was once considered a pathovar or sub-species of P. syringae, and in many places continues to be referred to as Pseudomonas syringae pv. savastanoi, although as a result of DNA-relatedness studies it has been instated as a new species. It itself has three host-specific pathovars, fraxini which causes ash canker, nerii which attacks oleander and oleae which causes olive knot.

Genome sequencing projects
The genomes of several strains of P. syringae have been sequenced, including P. syringae pv. tomato DC3000, P. syringae pv. syringae B728a and P. syringae pv. phaseolicola 1448A.