Pyrophosphate dependent phosphofructokinase

Pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase (EC 2.7.1.90, PFP) is an exclusively cytosolic enzyme that catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate in the glycolytic direction, and the de-phosphorylation of fructose-1,6-bisphoshate to fructose-6-phosphate in the gluconeogenic reaction. Reeves et al. (1974) first isolated PFP from Entamoeba histolytica, a lower eukaryote. The first plant PFP isolated was from the leaves of pineapples by Carnal and Black (1979) and it has since been isolated from a variety of plant species and tissues (Stitt 1990).