Enoyl-CoA hydratase

Enoyl-CoA hydratase is the enzyme used to catalyze the second step of β-oxidation in Fatty acid metabolism. It catalyzes the following reaction:



The reaction hydrates the bond between C-2 and C-3 on trans-Δ2-enoyl-CoA. The end-product is L-3-Hydroxyacyl CoA.

The reaction is a stereospecific syn-addition of water to a double bond, forming the L-isomer. The enzyme will also catalyze the hydration of a cis bond, as found in unsaturated fatty acids, but the isomer formed is then a D-isomer, which is not a substrate for the following steps of Fatty acid metabolism.

The enzyme is mechanistically similar to fumarase.

It is classified as.