TBE

Purpose

 * TBE is used for electrophoresis of nucleic acids in sequencing PAA gels or agarose gels.

Recipe
10x TBE (1 liter): Note: 10x TBE may take some time to dissolve, even with fast stirring
 * 1) Dissolve 108 g Tris and 55 g Boric acid in 900 ml distilled water.
 * 2) Add 40 ml 0.5 M Na2EDTA (pH 8.0) (alternatively use 9.3 g Na2EDTA)
 * 3) Adjust volume to 1 Liter.
 * 4) Store at room temperature.

1x TBE (1 liter):
 * 1) Dissolve 10.8 g Tris and 5.5 g Boric acid in 900 ml distilled water.
 * 2) Add 4 ml 0.5 M Na2EDTA (pH 8.0)
 * 3) Adjust volume to 1 Liter.
 * 4) Store at room temperature.

Commercial sources

 * 1) UltraPure™ 10X TBE Buffer from Invitrogen