HindIII

HindIII is a nuclease isolated from Haemophilus influenzae.

In molecular biology, it is commonly used as a restriction enzyme.

Recognition sequence of HindIII:

5'AAGCTT 3'TTCGAA

How HindIII cuts this sequence:

5'---A    AGCTT---3' 3'---TTCGA    A---5'

HindIII HindIII HindIII