Scatchard equation

The Scatchard equation is an equation for calculating the affinity constant of a ligand with a protein. The Scatchard equation is given by (r/c) = Ka*n - Ka*r, where r is the ratio of the concentration of bound ligand to total available binding sites, c is the concentration of free ligand, and n is the number of binding sites per antibody molecule.

Ka is the association (affinity) constant from the equation Ka = [Ab-Ag]/([Ab][Ag]), where Ab is the binding site on the antibody, Ag is a monovalent antigen, and Ag-Ab is antigen-bound antibody.

The Scatchard equation is sometimes referred to as the Rosenthal-Scatchard equation.

Plotting this data yields the Scatchard plot.

The Scatchard equation is named after the former MIT Chemistry Department member George Scatchard, an American chemist, 1892–1973.

See

 * some explanation
 * explanation
 * derivation

Scatchard-Diagramm