Enolase

Enolase (enol + suffix -ase) or 2-phospho-D-glycerate hydrolyase is an enzyme that participates in glycolysis.

Enolase catalyzes the conversion of 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP), the penultimate step in the conversion of glucose to pyruvate.

Isozymes
In humans there are five distinct enolase isozymes.
 * Alpha-enolase is ubiquitous in the cytoplasm of cells.
 * Beta is associated with muscle tissue.
 * Gamma is neuronal specific.

A type of enolase in glycolysis is phosphopyruvate hydratase.

Enolase superfamily
The enzyme is an archetypal member of an enzyme superfamily (the enolase superfamily), whereby a group of evolutionarily related enzymes share similar reaction mechanisms but function on different substrates (with altered substrate affinity) and in different biochemical context.

Inhibition
Enolase is inhibited by fluoride.