Honey bee pheromones

Honey bee pheromones are mixtures of chemical substances released by individual bees into the hive or environment that cause changes in the physiology and behaviour of other bees.

Introduction
Honeybees have one of the most complex pheromonal communication systems found in nature, possessing 15 known glands that produce an array of compounds. Pheromones are produced as a liquid and transmitted by direct contact as a liquid or as a vapor. Pheromones may be volatile or non-volatile.

The pheromones are chemical messengers secreted by a queen, drone, worker bee or laying worker bee that elicit a response in other bees. The chemical messages are received by the bee's antenna and other body parts. Honey bee (Apis mellifera) pheromones can be grouped into releaser pheromones with short term effects and primer pheromones with long term effects.

Primer pheromones change the physiology of the recipient. Releaser pheromones change the behavior of the recipient. Releaser pheromones have a short term effect and they trigger an almost immediate behavioral response from the receiving bee. Under certain conditions a pheromone can act as both a releaser and primer pheromone.

Pheromones are NOT single chemicals, but rather a complex mixture of numerous chemicals in different percentages.

Alarm pheromone
Two main alarm pheromones have been identified in honeybee workers. One is released by the Koschevnikov gland, near the sting shaft, and consists of more than 40 chemical compounds, including isopentyl acetate (IPA), butyl acetate, 1-hexanol, 1-butanol, 1-octanol, hexyl acetate, octyl acetate, n-pentyl acetate and 2-nonanol. These chemical compounds have low molecular weights, are highly volatile, and appear to be the least specific of all pheromones. Alarm pheromones are released when a bee stings another animal, and attract other bees to the location and causes the other bees to behave defensively, i.e. sting or charge. Smoke can mask the bees' alarm pheromone. The other alarm pheromone is released by the mandibular glands and consists of 2-heptanone, which is also a highly volatile substance. This compound has a repellent effect and it was proposed that it is used to deter potential ennemies and robber bees. Interestingly, the amounts of 2-heptanone increase with the age of bees and becomes higher in the case of foragers. It was therefore suggested that 2-heptanone is used by foragers to scent-mark recently visited and depleted foragers, which indeed are avoided by foraging bees.

Brood recognition pheromone
Another pheromone is responsible for preventing worker bees from bearing offspring in a colony that still has developing young. Both larvae and pupae emit a "brood recognition" pheromone. This inhibits ovarian development in worker bees and helps nurse bees distinguish worker larvae from drone larvae and pupae. This pheromone is a ten-component blend of fatty-acid esters, which also modulates adult caste ratios and foraging ontogeny dependent on its concentration. The components of brood pheromone have been shown to vary with the age of the developing bee.

Drone pheromone
Drones produce a pheromone that attracts other flying drones to promote drone aggregations at sites suitable for mating with virgin queens.

Dufour's gland pheromone
The Dufour’s gland opens into the dorsal vaginal wall. Dufour’s gland and its secretion have been somewhat of a mystery. The gland secretes its alkaline products in to the vaginal cavity, and it has been assumed to be deposited on the eggs as they are laid. Indeed, Dufour’s secretions allow worker bees to distinguish between eggs laid by the queen, which are attractive, and those laid by workers. The complex of as many as 24 chemicals differs between workers in "queenright" colonies and workers of queenless colonies. In the latter, the workers’ Dufour secretions are similar to those of a healthy queen. The secretions of workers in queenright colonies are long-chain alkanes with odd numbers of carbon atoms, but those of egg-laying queens and egg-laying workers of queenless colonies also include long chain esters.

Egg marking pheromone
This pheromone, similar to that described above, helps nurse bees distinguish between eggs laid by the queen bee and eggs laid by a laying worker.

Footprint pheromone
This pheromone is left by bees when they walk and is useful in enhancing Nasonov pheromones in searching for nectar.

In the queen, it is an oily secretion of the queen's tarsal glands that is deposited on the comb as she walks across it. This inhibits queen cell construction (thereby inhibiting swarming), and it production diminishes as the queen ages.

Forager pheromone
Ethyl oleate is released by older forager bees to slow the maturing of nurse bees. This primer pheromone acts as a distributed regulator to keep the ratio of nurse bees to forager bees in the balance that is most beneficial to the hive.

Nasonov pheromone
This pheromone is emitted by the worker bees and used for orientation.

Other pheromones
Other pheromones produced by most honeybees include rectal gland pheromone, tarsal pheromone, wax gland and comb pheromone, and tergite gland pheromone.

Queen mandibular pheromone (QMP)
QMP, emitted by the queen, is one of the most important sets of pheromones in the bee hive. It affects social behaviour, maintenance of the hive, swarming, mating behaviour, and inhibition of ovary development in worker bees. The effects can be short and/or long term. Some of the chemicals found in QMP are carboxylic acids and aromatic compounds. The following compounds have been shown to be important in retinue attraction of workers to their queen (Slessor, 1988) and other effects.
 * (E)-9-oxodec-2-enoic acid  (9-ODA)  - inhibits queen rearing as well as ovarian development in worker bees; strong sexual attractant for drones when on a nuptial flight; critical to worker recognition of the presence of a queen in the hive
 * (R,E)-(-)-9-hydroxy-2-enoic acid    (9-HDA)  promotes stability of a swarm, or a "calming" influence
 * (S,E)-(+)-9-HDA
 * Methyl-p-hydroxybenzoate (HOB)
 * 4-hydroxy-3-methoxy phenylethanol (HVA)

Synthetic queen mandibular pheromone (QMP) is a mixture of five components 9-ODA, (-) isomer (9-HDA), (+) isomer of (9-HDA), HOB and HVA in a ratio of 118:50:22:10:1.

Queen retinue pheromone (QRP)
The following compounds have also been identified, of which only coniferyl alcohol is found in the mandibular glands. The combination of the 5 QMP compounds and the 4 compounds below is called the Queen Retinue Pheromone (QRP). These nine compounds are important for the retinue attraction of worker bees around their queen.
 * methyl (Z)-octadec-9-enoate (methyl oleate)
 * (E)-3-(4-hydroxy-3-methoxyphenyl)-prop-2-en-1-ol (coniferyl alcohol)
 * hexadecan-1-ol
 * (Z9,Z12,Z15)-octadeca-9,12,15-trienoic acid (linolenic acid)

References listed alphabetically by author

 * Imrie, George Georg Imrie's, Pink Pages Nov. 1999
 * Katzav-Gozansky, Tamar Apidologie 33 (2002) 525–537


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 * Imrie, George George Imrie's Pink Pages November 1999 accessed Feb. 2005
 * Keeling, C. I., Slessor, K. N., Higo, H. A. and Winston, M. L. (2003) Isolation and identification of new components of the honey bee (Apis mellifera L.) queen retinue pheromone. Proc National Academy of Science USA 100: 4486-4491.
 * Leoncini, I., Le Conte, Y., Costagliola, G., Plettner, E., Toth, A. L., Wang, M., Huang, Z., Bécard, J.-M., Crauser, D., Slessor, K. N. and Robinson, G. E. (2004) Regulation of behavioral maturation by a primer pheromone produced by adult worker honey bees. Proc Natl Acad Sci USA 101: 17559-17564.
 * Maschwitz, U., 1964. Alarm substances and alarm behavior in social Hymenoptera, Nature 204, 324-327.
 * Moritz, R.F.A. and H. Burgin. 1987. Group response to alarm pheromones in socialwasps and the honeybees. Ethology 76, 15-26
 * Slessor, K. N., Kaminski, L.-A., King, G. G. S., Borden, J. H. and Winston, M. L. (1988) Semiochemical basis of the retinue response to queen honey bees. Nature 332: 354-356.
 * Vander Meer, R.K. et al. 1998. Pheromone Communication in Social Insects; Boulder: Westview Press
 * Wager, B.R. and M.D. Breed. 2000. Does honeybee sting alarm pheromone give orientation information to defensive bees? Annals of the Entomological Society of America 93(6), 1329-1332