CLED agar

CLED agar (Cysteine Lactose Electrolyte Deficient medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary organisms. Being electrolyte deficient, it prevents the swarming of Proteus species. Lactose fermenters produce yellow colonies on CLED agar; non-lactose fermenters appear blue. It has a pH of approximately 7.3. CLED agar contains:

{| class="wikitable"
 * Peptone
 * 4g/l
 * 'Lab Lemco' powder
 * 3g/l
 * Tryptone
 * 4g/l
 * Lactose
 * 10g/l
 * L-Cysteine
 * 128mg/l
 * Bromothymol blue
 * 20mg/l
 * Agar No. 1
 * 15g/l
 * Bromothymol blue
 * 20mg/l
 * Agar No. 1
 * 15g/l
 * Agar No. 1
 * 15g/l