Cholesterol oxidase

In enzymology, a cholesterol oxidase is an enzyme that catalyzes the chemical reaction


 * cholesterol + O2 $$\rightleftharpoons$$ cholest-4-en-3-one + H2O2

Thus, the two substrates of this enzyme are cholesterol and O2, whereas its two products are cholest-4-en-3-one and H2O2.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

Structural studies
As of late 2007, 14 structures have been solved for this class of enzymes, with PDB accession codes, , , , , , , , , , , , , and.