Explant culture

Explant culture is a technique used for the isolation of cells from a piece or pieces of tissue.

In brief, the tissue is harvested in a sterile manner, often minced, and pieces placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue onto the surface of the dish. These primary cells can then be further expanded and transferred into fresh dishes.

Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture.