Tetraacyldisaccharide 4'-kinase

In enzymology, a tetraacyldisaccharide 4'-kinase is an enzyme that catalyzes the chemical reaction


 * ATP + [2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl]-(1->6)-[2- N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate] $$\rightleftharpoons$$ ADP + [2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D- glucosaminyl]-(1->6)-[2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D- glucosaminyl phosphate]

The 3 substrates of this enzyme are ATP, [2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl]-(1->6)-[2-, and N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate], whereas its 4 products are ADP, [2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-, glucosaminyl]-(1->6)-[2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-, and glucosaminyl phosphate].

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:2,3,2',3'-tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-beta-D -1,6-glucosaminyl-beta-phosphate 4'-O-phosphotransferase. This enzyme is also called lipid-A 4'-kinase. This enzyme participates in lipopolysaccharide biosynthesis.

Structural studies
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code.