Fosmid

Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E.coli) can only contain one fosmid molecule. Low copy number offers higher stability than comparable high copy number cosmids. Fosmid clones were used to help assess the accuracy of the Public Human Genome Sequence. Ung-Jin Kim et al. 1992. Stable propagation of cosmid-sized human DNA inserts in an F-factor based vector. Nucleid Acids Res. 20:1083-1085.