Calcium imaging

Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium status of a tissue or medium. Due to the chemical properties of calcium it can not be imaged easily. Instead, another substance called Fura-2 fluoresces after binding to calcium and being exposed to a fluorescent light. The Fura-Ca complex affects the wavelength typically associated with unbound Fura-2 and the resulting fluorescence can be detected by a camera adapted (usually through a microscope) for fluorescent light imaging. An image is thus created which can be analyzed according to intensity, ultimately reflecting the Ca status.

Technical modifications
The traditional calcium imaging technique can also be modified to indicate a quenching of fluorescence via addition of manganese (Mn). The subsequent binding to Fura-2 by Mn quenches the fluorescence since the Fura-Mn complex does not share the same wavelength properties as Fura-Ca. It is also necessary to adjust the detection wavelength since Fura is usually detected at 340 and 380 wavelengths. Mn quenching is best associated with the 360.