Public Health Genomics

Public Health Genomics is the utilization of genomics information to benefit public health. This is visualized as more effective personalised preventive care and disease treatments with better specificity, targeted to the genetic makeup of each patient. (Bellagio Group on Public Health Genomics).

According to the CDC, Public Health genomics is an emerging field that assesses the impact of genes and their interaction with behavior, diet and the environment on the population’s health. (IOM 2005- http://www.cdc.gov/genomics/activities/file/print/infosharing_khoury.pdf)

This field of public health is less than a decade old. A number of think tanks, universities, and governments (including the U.S., UK, and Australia) have started public health genomics projects. Research on the human genome is generating new knowledge that will require changes in public health programs and policies. As advances in genomic sciences are increasingly used to improve health and prevent disease, educating and training the public health workforce, other healthcare providers, and citizens will be paramount to integrating this new knowledge into existing public health programs. In particular, providing genomic education to—and with—the public will help protect consumers against premature or inappropriate uses of genetic testing. Both the complexity and sheer amount of information related to genomics and health care may be overwhelming to both health care practitioners and the lay public. As new strategies, products and services evolve from genomic technologies in the coming years, it will become increasingly necessary to provide both specialized genomics-related education and training and general public information to enhance awareness, build competencies, make informed decisions, and ensure continuity of access to health services.

Advancing public health
The publication of the sequence of the human genome only fifty years following the discovery of DNA promises to be a scientific landmark in biological research and more specifically, in clinical Internal Medicine. In prior periods of medical history, a lack of educational tools slowed the pace of clinical progress related to profound advances in the medical sciences. In the current era, education must partner with further scientific discovery to hasten the potential of the human genome sequence. Specific tools are already developed to create a new genetic/scientific literacy that is necessary to translate these tools to the bedside and to engage the crucial large population studies that are needed to examine both the promise and perils of the application of information generated by research on the human genome. Without strong continuing education programs both within and outside the university environment, it will be impossible to close the workforce knowledge gap in genomics. Public education is a critical part of that effort, but it has fallen short, primarily because public health professionals are still in the process of educating themselves. Ultimately, therefore, resources will be needed to mount the kind of effort necessary to assure that genomics is used properly for the benefit of the public's health. For example, more must be learned about the general language and terms that can make a difference to the public's understanding of genetics, particularly, an improved understanding of how probabilistic concepts can best be conveyed. For example, what are the effects of being told that one has a genetic predisposition for a disease?

Putting the "public" in public health
Genomic information is deeply relevant to each member of society, and as the science progresses, major efforts must be made to involve public in that research and subsequent policy. Opinions vary widely on the means by which the public can be involved, but a trend is emerging toward partnerships between communities and scientists, with the results being disseminated to policymakers. Some issues over which the public has a major stake include: "banks" of genetic information taken at birth and stored by governments (length of time genetic information is kept before being destroyed varies on a state-by-state level in the U.S.); discrimination by employers and insurers based on access to personal genomic information; access to technology that makes personalized genetic information available to patients. See geneforum.com for more information.

Public policy has an important role in protecting against genetic discrimination, defined in Taber's Cyclopedic Medical Dictionary (2001) as unequal treatment of persons with either known genetic abnormalities or the inherited propensity for disease; genetic discrimination may have a negative effect on employability, insurability and other socio-economic variables. Public policy in the U.S. to protect individuals and groups of people against genetic discrimination include the Americans with Disabilities Act (1990)ADA, Executive Order 13145 (2000) that prohibits genetic discrimination in the workplace for federal employees, and the Genetic Information Nondiscrimination Act (2007, first introduced in 2003).

Main public concerns in genomic information are that of (1) Confidentiality (2) Misuse of information (Discrimination by health plans, employer, and medical practitioners) (3) Right and access to genetic information. It is important to cater to these concerns while evolving policies to further the cause of genomics and its application. Progress in public health genomics will slow down without large-scale community participation. It requires involvement of diseased members, members at high risk and people at low risk to understand the interaction between genes and the environment and its consequence. This involvement needs to be encouraged guarding the individual interests of the citizens. Involving public, by educating them about this emerging field, in policymaking can booster confidence in science, facilitate research and its application.

Ethical concerns
One of the many facets involved in Public Health Genomics is the ethical area. This has recently been highlighted in a study by Cogent Research (http://www.redorbit.com/news/display/?id=294054) that found when American citizens were asked what they thought the strongest drawback was in using genetic information, they listed "misuse of information/invasion of privacy" as the single most important problem. With such a large proportion of the population acknowledging this as a major pitfall, this must be one of the areas mediated in any discourse between the public and healthcare officials. Although no legislation has been passed limiting the use of genetic discrimination in individual’s insurance coverage or to genetic discrimination in the workplace, in 2000 President Bill Clinton signed an executive order banning federal agencies from using genetic information to discriminate against potential applicants. Furthermore, The Genetic Information Nondiscrimination Act of 2007 has passed the House of Representatives and is currently been referred to the Senate Committee on Health, Education, Labor, and Pensions (June, 2007). For up to date news visit the Genetics Privacy and Legislation homepage at "http://www.ornl.gov/sci/techresources/Human_Genome/elsi/legislat.shtml".

In order for the information obtained to benefit the public health a number of key bioethical principles must be considered. Although the information will be beneficial to the public health, the role of the individual must not be forgotten. If it were not the for the information gleaned off of the individual, this discussion would probably not be taking place. Why would someone have a genetic test to tell them if they were going to come down with a disease that has no treatment? Some people would follow the utilitarian ideal and have the test to aid in the advancement of scientific technology so one day there might be a treatment. In order to afford this act some protection the individual must not become lost or sacrificed to the good of the populous. The first principle that must be acknowledged is the respect for the person as an autonomous agent. If one acknowledges the individuals right to make the best choices for their healthcare, then that same acknowledgment should extend to the respect for their choices. Not only must the individual be respected, the amount of the other people effected by the situation should be maximized. Once this information has been obtained there needs to be a sense of confidentiality. This must be done in order to retain professional integrity and, therefore, lead to future gathering of information. Without the respect for the profession, the progression of the science will come to a halt. Finally, professionals dealing with this information must keep in mind the principles of beneficence and nonmaleficence. The principles of nonmaleficence and beneficence should also be applied according to distributive justice. That is we must not just focus on the utilitarian good that will come of this information. Instead we must keep in mind the Hippocratic Oath and try to maximize the benefit while minimizing the the harm to all parties involved. Just because the potential benefit might be great does not mean that we should always proceed.

In 2003, the Nuffield Council on Bioethics published a report, "Pharmacogenetics: Ethical Issues". Authors of the document explore four broad categories of ethical and policy issues related to pharmacogenetics: information, resource, equity and control. In the introduction to the report, the authors clearly state that the development and application of pharmacogentics depend on scientific research, but that policy and administration must provide incentives and restraintsto ensure the most productive and just use of this technology [15].

Genetic susceptibility to disease
There are many ways by which genes may account for disease. Single nucleotide polymorphisms (SNPs) are one of the most important single base changes (mutations) in the nucleotide sequence of one's genome. They may have no effect at all on gene expression, or they can change the function of a gene completely. Although mutations occur throughout the body, only mutation in the sex cells of males and females will perpetuate the mutation to the next generation of individuals. Some current tests for genetic diseases include: Cystic Fibrosis, Tay Sachs Disease, Lou Gehrig’s Disease (ALS), Huntington’s Disease, catastrophically high cholesterol, some rare cancers, inherited susceptibility to cancer. A select few are explored below.

Herpesvirus and bacterial infections
Since the field of genomics takes into account the entire genome of an organism and not simply its chromosomes, the presence of latent viral infection falls into this realm. For example, the DNA of a latent Herpesvirus combines with the host’s chromosome and propagates through cell replication, even though it is not a part of the chromosome and was not present at the birth of the organism. On 17 May 2007, Nature  published a study that showed mice with a latent infection of a Herpesvirus were less susceptible to bacterial infections. Mice were infected with murine gammherpesvirus 68 (a member of the gammaherpesvirinae subfamily) and then challenged with Listeria monocytogenes. Mice that had a latent infection of the virus had an increased resistance to the bacteria, but those with a non-latent strain of virus did not change susceptibility to the bacteria.

The study went on to test mice with murine cytomegalovirus, a member of the betaherpesvirinae subfamily, which provided similar results. However, infection with human herpes simplex virus type-1, a member of the alphaherpesvirinae subfamily, did not provide increased resistance to bacterial infection. They also used Yersinia pestis (the causative agent of the Black Death) to challenge mice with a latent infection of gammaherpesvirus 68, and they found the mice did have an increased resistance to the bacteria. The suspected reason for this is that peritoneal macrophages in the mouse are activated after latent infection of the herpesvirus, and since macrophages play an important role in immunity, this provides the mouse with a stronger, active immune system at the time of bacterial exposure. It was found that the latent herpesvirus caused an increase in interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α), both of which lead to activation of macrophages and resistance to bacterial infection.

Since most, if not all, humans are exposed to herpesviruses throughout their lifetime (especially childhood), it would be interesting to know if latent herpesvirus infections in humans also provide an enhanced resistance to bacterial infection. If this is true, then our current concepts of immunology as it pertains to resisting bacterial infections would need to be recalculated to consider the additional herpesvirus DNA in our genomes.

Influenza and Mycobacterium tuberculosis
Variations within the human genome can be and are studied to determine susceptibility to infectious diseases. The study of variations within microbial genomes will also need to be evaluated to utilize genomics of infectious disease within public health. The ability to determine if a person has greater susceptibility to an infectious disease will be valuable to determine how to treat the disease if it is present or prevent the person from getting the disease. Several infectious diseases have shown a link between genetics and susceptibility in that families tend to have heritability traits of a disease.

During the course of the past influenza pandemics and the current influenza epizootic there has been evidence of family clusters of disease. Kandun, et. al. found that family clusters in Indonesia in 2005 resulted in mild, severe and fatal cases among family members. The findings from this study raise questions about genetic or other predispositions and how they affect a persons susceptibility to and severity of disease. Continued research will be needed to determine the epidemiology of H5N1 infection and whether genetic, behavioral, immunologic, and environmental factors contribute to case clustering.

Host genetic factors play a major role in determining differential susceptibility to major infectious diseases of humans. Infectious diseases of humans appear highly polygenic with many loci implicated but only a minority of these convincingly replicated. Over the course of time humans have been exposed to organisms like Mycobacterium tuberculosis. It is possible that the human genome has evolved in part from our exposure to M. tuberculosis. Animal model studies and whole genome screens can be used to identify potential regions on a gene that suggest evidence of tuberculosis susceptibility. In the case of M. tuberculosis, animal model studies were used to suggest evidence of a locus which was correlated with susceptibility, further studies were done to prove the link between the suggested locus. The genetic loci that have been identified to be associated with susceptibility to tuberculosis are HLA-DR, INF-γ, SLC11A1, VDR, MAL/TIRAP, and CCL2. Further studies will be needed to determine genetic susceptibility to other infectious diseases and ways public health officials can prevent and test for these infections to enhance the concept of personalized medicine.

Type 1 Diabetes, immunomics, and public health
The term genomics, referring to the organism’s whole genome, is also used to refer to gene informatics, or the collection and storage of genetic data, including the functional information associated with the genes, and the analysis of the data as combinations, patterns and networks by computer algorithms. Systems biology and genomics are natural partners, since the development of genomic information and systems naturally facilitates analysis of systems biology questions involving relationships between genes, their variants (SNPs) and biological function. Such questions include the investigation of signaling pathways, evolutionary trees, or biological networks, such as immune networks and pathways. For this reason, genomics and these approaches are particularly suited to studies in immunology. The study of immunology using genomics, as well as proteomics and transcriptomics (Includes gene profiles, either genomic or expressed gene mRNA profiles), has been termed immunomics.

Accurate and sensitive prediction of disease, or detection during early stages of disease, could allow the prevention or arrest of disease development as immunotherapy treatments become available. Type 1 diabetes markers associated with disease susceptibility have been identified, for example HLA class II gene variants, however possession of one or more of these genomic markers does not necessarily lead to disease. Lack of progression to disease is likely due to the absence of environmental triggers, absence of other susceptibility genes, presence of protective genes, or differences in the temporal expression or presence of these factors. Combinations of markers have also been associated with susceptibility to type 1 diabetes however again, their presence may not always predict disease development, and conversely, disease may be present without the marker group. Potential variant genes (SNPs) or markers that are linked to the disease include genes for cytokines, membrane-bound ligands, insulin and immune regulatory genes.

Metaanalyses have been able to identify additional associated genes [16], by pooling a number of large gene datasets. This successful study illustrates the importance of compiling and sharing large genome databases. The inclusion of phenotypic data in these databases will enhance discovery of candidate genes, while the addition of environmental and temporal data should be able to advance the disease progression pathways knowledge. HUGENet, or Human Genome Epidemiology Network, which was initiated by the CDC, is accomplishing the integration of this type of information with the genome data, in a form available for analysis[17]. This project could be thought of as an example of ‘metagenomics’, the analysis of a community’s genome [18], but for a human rather than a microbial community. This project is intended to promote international data sharing and collaboration, in addition to creating a standard and framework for the collection of this data. This year the CDC funded a number of studies, Seed CDC Funding for Public Health Genomics Research, to integrate public health and genomics information, although this year none of the funded studies focused type 1 diabetes.

Nonsyndromic hearing loss
Variations within the Genome are being studied to determine susceptibility to chronic diseases as well as infectious diseases. According to Aileen Kenneson and Coleen Boyle, about one sixth of the U.S. population has some degree of hearing loss[10]. Recent research has linked variants in the Gap Junction Beta 2 (GJB2) gene to nonsyndromic prelingual sensorineural hearing loss. GJB2 is a gene encoding for connexin a protein found in the cochlea. So far, scientists have found over 90 variants in this gene and sequence variations may account for up to 50% of nonsyndromic hearing loss. Variants in GJB2 are being looked at to determine age of onset as well as severity of hearing loss.

It is clear that there are also environmental factors to consider. Infections such as rubella and meningitis, low birth weight and ventilator use, are known risk factors for hearing loss, but perhaps knowing this as well as genetic information will help with early intervention.

Information gained from further research in the role of GJB2 variants in hearing loss may lead to consisted newborn screening for them. As early intervention is crucial to prevent developmental delays in children with hearing loss, the ability to test for susceptibility in young children would be beneficial. Knowing genetic information may also help in the treatment of other diseases if a patient is already at risk.

Further testing is needed, especially in determining the role of GJB2 variants and environmental factors on a population level, however initial studies show promise when using genetic information along with newborn screening.

Pharmacogenomics
The Word Health Organization has defined pharmacogenomics pharmacogenomics as the study of DNA sequence variation as it relates to differential drug response in individuals, i.e., the use of genomics to determine an individual’s response. Pharmacogenomics refers to the use of DNA-based genotyping in order to target pharmaceutical agents to specific patient populations in the design of drugs [15].

Current estimates state that 2 million hospital patients are effected by adverse drug reactions every year and adverse drug events are the fourth leading cause of death. These adverse drug reactions result in an estimated economic cost of $136 billion per year. Polymorphisms (genetic variations) in individuals effect drug metabolism and therefore an individual's response to a medication. Examples of ways in which genetics may effect an individual’s response to drugs include: drug transporters, metabolism and drug interactions. Pharmacogenetics may be used in the near future by public health practitioners to determine the best candidates for certain drugs, thereby reducing much of the guesswork in prescribing drugs. Such actions have the potential to improve the effectiveness of treatments and reduce adverse drug events.

Nutrition and Health
Nutrition is very important in determining various states of health. The field of nutrigenomics based on the idea that everything ingested into a person’s body affect the genome of the individual. This may be through either upregulating or downregulating the expression of certain genes or by a number of other methods. While the field is quite young there are a number of companies that market directly to the public and promote the issue under the guise of public health. Yet many of these companies claim to benefit the consumer, the tests performed are either not applicable or often result in common sense recommendations. Such companies promote public distrust towards future medical tests that may test more appropriate and applicable agents.

An example of the role of nutrition would be the methylation pathway involving methylene tetrahydrofolate reductase (MTHFR). An individual with the gene variant or SNP (single nucleotide polymorphism) may need increased intake of B12 and Folinic acid to override the effect of a variant SNP. While MTHFR variants c677T are common affecting up to 30% of the population, certain populations such as those affected by autism, appear to carry the SNP for MTHFR, either homozygous or heterozygous (http://www.jpands.org/vol9no4/boris.pdf). Increased risk for neural tube defects (http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1469-1809.2003.00027.x) and elevated homocysteine levels have been associated with the MTHFR C677T polymorphism.(http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=11953142&dopt=Abstract

In 2002, researchers from the Johns Hopkins Bloomberg School of Public Health identified the blueprint of genes and enzymes in the body that enable sulforaphane, a compound found in broccoli and other vegetables, to prevent cancer and remove toxins from cells. The discovery was made using a “gene chip,” which allows researchers to monitor the complex interactions of thousands of proteins on a whole genome rather than one at time. This study was the first gene profiling analysis of a cancer-preventing agent using this approach [13]. University of Minnesota researcher Sabrina Peterson, PhD, RD, coauthored a study with Johanna Lampe of the Fred Hutchinson Cancer Research Center, Seattle, in October 2002 that investigated the chemoprotective effect of cruciferous vegetables (e.g., broccoli, Brussels sprouts). Study results published in The Journal of Nutrition outline the metabolism and mechanisms of action of cruciferous vegetable constituents, discusses human studies testing effects of cruciferous vegetables on biotransformation systems and summarizes the epidemiologic and experimental evidence for an effect of genetic polymorphisms (genetic variations) in these enzymes on response to cruciferous vegetable intake [14].

Healthcare and Genomics
How will obtaining my genetic blueprint benefit me? Why find that I am more susceptible to getting a disease that has no cure? These are relevant questions that are continually being asked by the public, as researchers continue to unfold the mystery of the human genome. It is important to ask these questions taking into consideration the current knowledge of genomics, in comparison to, the potential genomics will one day have on healthcare as we know it today.

Researchers have found that almost all disorders and diseases that affect humans reflect the interplay between the environment and their genes; however we are still in the initial stages of understanding the specific role genes play on common disorders and diseases.[11] For example, while news reports may give a different impression, most cancer is NOT inherited. It is therefore likely that the recent rise in the rates of cancer worldwide can be at least partially attributed to the rise in the number of synthetic and otherwise toxic compounds found in our society today. Thus, in the near future, public health genomics, and more specifically environmental health, will become an important part of the future healthcare-related issues.

Potential benefits of uncovering the human genome will be focused more on identifying causes of disease and less on treating disease, through: improved diagnostic methods, earlier detection of a predisposing genetic variation, pharmacogenomics and gene therapy.

For each individual knowing their genetic make-up will be different. For some individuals, they will be given the assurance of not obtaining a disease ,as a result of familial genes, in which their family has a strong history and some will be able to seek out better medicines or therapies for a disease they already have. Others will find they are more susceptible to a disease that has no cure. Though this information maybe painful, it will give them the opportunity to prevent or delay the on-set of that disease through: increased education of the disease, making lifestyle changes, finding preventive therapies or identifying environmental triggers of the disease. As we continue to have advances in the study of human genetics, we hope to one day incorporate it into the day-to-day practice of healthcare. Understanding your own genetic blueprint can empower you to take an active role in promoting your own health.

Genomics and understanding of disease susceptibility can help validate family history tool for use by practitioners and the public. IOM is validating the family history tool for six common chronic diseases(breast, ovarian, colorectal cancer, diabetes, heart disease, stroke)(IOM Initiative). Validating cost effective tools can help restore importance of basic medical practices (e.g. family history)in comparission to technology intensive investigations. (http://www.cdc.gov/genomics/activities/file/print/infosharing_khoury.pdf)

The genomic face of immune responses
A critical set of phenomena that ties together various aspects of health interventions, such as drug sensitivity screening, cancer or autoimmune susceptibility screening, infectious disease prevalence and application of pharmacologic or nutrition therapies, is the systems biology of the immune response. For example, the influenza epidemic of 1918, as well as the recent cases of human fatality due to H5N1 (avian flu), both illustrate the potentially dangerous sequence of immune responses to this virus. Also well documented is the only case of spontaneous "immunity" to HIV in humans, shown to be due to a mutation in a surface protein on CD4 T cells, the primary targets of the HIV virus. The immune system is truly a sentinel system of the body, with the result that health and disease are carefully balanced by the modulated response of each of its various parts, which then also act in concert as a whole. Especially in industrialized and rapidly developing economies, the high rate of allergic and reactive respiratory disease, autoimmune conditions and cancers are also in part linked to aberrant immune responses that are elicited as the communities' genomes encounter swiftly changing environments. The causes of perturbed immune responses run the gamut of genome-environment interactions due to diet, supplements, sun exposure, workplace exposures, etc. Public health genomics as a whole will absolutely require a rigorous understanding of the changing face of immune responses.

Newborn Screening
The experience of newborn screening serves as the introduction to public health genomics for many people. If they did not undergo prenatal genetic testing, having their new baby undergo a heel stick in order to collect a small amount of blood may be the first time an individual or couple encounters genetic testing. Newborn genetic screening is a promising area in public health genomics that appears poised to capitalize on the public health goal of disease prevention as a primary form of treatment.

Most of the diseases that are screened for are extremely rare, single-gene disorders that are often autosomal recessive conditions and are not readily identifiable in neonates without these types of tests. Therefore, often the treating physician has never seen a patient with the disease or condition and so an immediate referral to a specialty clinic is necessary for the family.

Most of the conditions identified in newborn screening are metabolic disorders that either involve i) lacking an enzyme or the ability to metabolize (or breakdown) a particular component of the diet, like phenylketonuria, ii) abnormality of some component of the blood, especially the hemoglobin protein, or iii) alteration of some component of the endocrine system, especially the thyroid gland. Many of these disorders, once identified, can be treated before more severe symptoms, such as mental retardation or stunted growth, set in.

Newborn genetic screening is an area of tremendous growth. In the early 1960s, the only test was for phenylketonuria. In 2000, roughly two-thirds of states in the US screened for 10 or fewer genetic diseases in newborns. Notably, in 2007, 95% of states in the US screen for more than 30 different genetic diseases in newborns. Especially as costs have come down, newborn genetic screening offers “an excellent return on the expenditure of public health dollars.” [11]

Understanding Traditional Healing Practices
Better understanding of genomics will help understand the practices that have evolved in older civilization over centuries and have been strengthen by observations (phenotype presentations) from generation to generation, but lack documentation and scientific evidence. Traditional healers have realized the importance of specific body types and its importance based on ability to succumb or resist particular diseases under specific conditions. It has not been possible to validate and standardize this knowledge/ practices in the world of modern science. Genomics could help open the door to scientific understanding of some traditional healing practices and help weed out unhealthy practices.

Resources and external links

 * National Office of Public Health Genomics (USA)
 * PHG Foundation (Foundation for Genomics and Population Health)
 * Cambridge Genetics Knowledge Park
 * Centers for Genomics and Public Health
 * Public Health Genomics European Network (PHGEN), financed by the European Commission (DG SANCO)
 * German Centre for Public Health Genetis
 * TOGEN – Turkish Centre for Public Health Genomics
 * P3G Consortium – Public Population Project in Genomics
 * Public Health Genomics Society [www.phgs.org]
 * GRaPH Int
 * Minnesota Gene Pool blog
 * U.S. Government - Genetics Privacy and Legislation Homepage
 * World Health Organization Genomic Resource Centre