(R)-6-hydroxynicotine oxidase

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In enzymology, a (R)-6-hydroxynicotine oxidase (EC is an enzyme that catalyzes the chemical reaction

(R)-6-hydroxynicotine + H2O + O2 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2

The 3 substrates of this enzyme are (R)-6-hydroxynicotine, H2O, and O2, whereas its two products are 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one and H2O2.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. The systematic name of this enzyme class is (R)-6-hydroxynicotine:oxygen oxidoreductase. Other names in common use include D-6-hydroxynicotine oxidase, and 6-hydroxy-D-nicotine oxidase. It employs one cofactor, FAD.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2BVF, 2BVG, and 2BVH.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
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  • Google Scholar references for
  • Bruhmuller M, Mohler H, Decker K (1972). "Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase". Eur. J. Biochem. 29: 143&ndash, 51. PMID 4628374.
  • Decker K, Bleeg H (1965). "Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans". Biochim. Biophys. Acta. 105: 313&ndash, 24. PMID 5849820.

External links

The CAS registry number for this enzyme class is 37233-46-8.

Gene Ontology (GO) codes