- acetyl-CoA + 3-methyl-2-oxobutanoate + H2O (2S)-2-isopropylmalate + CoA
This enzyme belongs to the family of transferases, specifically those acyltransferases that convert acyl groups into alkyl groups on transfer. The systematic name of this enzyme class is acetyl-CoA:3-methyl-2-oxobutanoate C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming). Other names in common use include 3-carboxy-3-hydroxy-4-methylpentanoate 3-methyl-2-oxobutanoate-lyase, (CoA-acetylating), alpha-isopropylmalate synthetase, alpha-isopropylmalate synthase, alpha-isopropylmalic synthetase, isopropylmalate synthase, and isopropylmalate synthetase. This enzyme participates in valine, leucine and isoleucine biosynthesis and pyruvate metabolism. It employs one cofactor, potassium.
- IUBMB entry for 188.8.131.52
- BRENDA references for 184.108.40.206 (Recommended.)
- PubMed references for 220.127.116.11
- PubMed Central references for 18.104.22.168
- Google Scholar references for 22.214.171.124
- Kohlhaw G, Leary TR, Umbarger HE (1969). "Alpha-isopropylmalate synthase from Salmonella typhimurium Purification and properties". J. Biol. Chem. 244: 2218&ndash, 25. PMID 4976555.
- Webster RE and Gross, SR (1965). "The alpha-isopropylmalate synthetase of Neurospora. I. The kinetics and end product control of alpha-isopropylmalate synthetase function". Biochemistry. 4: 2309&ndash, 2327.
- Cole FE, Kalyanpur MG, Stevens CM (1973). "Absolute configuration of alpha isopropylmalate and the mechanism of its conversion to beta isopropylmalate in the biosynthesis of leucine". Biochemistry. 12: 3346&ndash, 50. PMID 4270046.
- The CAS registry number for this enzyme class is 9030-98-2.
Gene Ontology (GO) codes
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