3alpha-hydroxysteroid dehydrogenase (B-specific)

Jump to: navigation, search

In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction

androsterone + NAD(P)+ 5alpha-androstane-3,17-dione + NAD(P)H + H+

The 3 substrates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases. The systematic name of this enzyme class is 3alpha-hydroxysteroid:NAD(P)+ oxidoreductase (B-specific). Other names in common use include hydroxyprostaglandin dehydrogenase, 3alpha-hydroxysteroid oxidoreductase, and sterognost 3alpha. This enzyme participates in 3 metabolic pathways: bile acid biosynthesis, c21-steroid hormone metabolism, and androgen and estrogen metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1AFS, 1FJH, 1FK8, 1LWI, 1RAL, 2DKN, and 2FVL.

References

  • IUBMB entry for 1.1.1.50
  • BRENDA references for 1.1.1.50 (Recommended.)
  • PubMed references for 1.1.1.50
  • PubMed Central references for 1.1.1.50
  • Google Scholar references for 1.1.1.50
  • JARABAK J, TALALAY P (1960). "Stereospecificity of hydrogen transfer by pyridine nucleotide-linked hydroxysteroid dehydrogenases". J. Biol. Chem. 235: 2147&ndash, 51. PMID 14406805.
  • KOCHAKIAN CD, CARROLL BR, UHRI B (1957). "Comparisons of the oxidation of C19-hydroxysteroids by guinea pig liver homogenates". J. Biol. Chem. 224: 811&ndash, 8. PMID 13405910.
  • Marcus PI and Talalay P (1956). "Induction and purification of alpha- and beta-hydroxysteroid dehydrogenases". J. Biol. Chem. 218: 661&ndash, 674.
  • Penning TM, Sharp RB (1987). "Prostaglandin dehydrogenase activity of purified rat liver 3 alpha-hydroxysteroid dehydrogenase". Biochem. Biophys. Res. Commun. 148: 646&ndash, 52. PMID 3479982.

External links

The CAS registry number for this enzyme class is 9028-56-2.

Gene Ontology (GO) codes


Linked-in.jpg