Bovine serum albumin
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Overview
Bovine serum albumin, Bovine Albumin, BSA: A serum albumin protein that can be used as a diluent or a blocking agent in numerous applications including ELISAs (Enzyme-Linked Immunosorbent Assay), blots and immunohistochemistry. It is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost since it is readily available in large quantities as it is purified from bovine blood, a byproduct of the beef industry.
Physical properties
- Number of residues: 583
- Molecular Weight: 66.430 kDa
- pI in Water at 25 ℃: 4.7
- Extinction Coefficient: 0.6 ml mg-1 cm-1
A 18-residue signal peptide is cut off from the precursor protein upon secretion, hence the precursor has 607 amino acid residues and a molecular weight of 69.3234 kDa.
References
- The sequence of BSA on NCBI.
- Product Sheet Information at Sigma-Aldrich
- Largest manufacturer of BSA: Proliant
See also
External links
 | This protein-related article is a stub. You can help WikiDoc by expanding it. |
Proteins: albumin | |
|---|---|
| Egg white (albumEn) | Conalbumin - Ovalbumin - Avidin |
| Serum albumin | Human serum albumin - Bovine serum albumin - Prealbumin |
| Other | C-reactive protein - Lactalbumin - Parvalbumin - Ricin |
Acknowledgement and Attribution Regarding Sources of Content
Some of the initial content on this page may be incorporated in part from copyleft sources in the public domain including wikis such as Wikipedia and AskDrWiki. Drug information for patients came from the The National Library of Medicine. Infectious disease information may have come from the Centers for Disease Control (CDC). Differential Diagnoses are drawn from clinicians as well as an amalgamation of 3 sources: 1.The Disease Database; 2. Kahan, Scott, Smith, Ellen G. In A Page: Signs and Symptoms. Malden, Massachusetts: Blackwell Publishing, 2004:3; 3. Sailer, Christian, Wasner, Susanne. Differential Diagnosis Pocket. Hermosa Beach, CA: Borm Bruckmeir Publishing LLC, 2002:7 .
