Calcein
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| Calcein | |
|---|---|
| Image:Calcein.png | |
| Identifiers | |
| CAS number | |
| SMILES | O=C(O5)c1ccccc1C (c3cc(CN(CC(O)=O) CC(O)=O)c(O)cc3O4) 5c2c4cc(O)c(CN(CC (O)=O)CC(O)=O)c2 |
| Properties | |
| Molecular formula | C30H26N2O13 |
| Molar mass | 622.55 g/mol |
| Melting point |
Decomposes |
| Boiling point |
N/A |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references | |
Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with an excitation and emission wavelengths of 495/515nm, respectively. Calcein also self-quenches at concentrations above 100mM. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. It has the appearance of orange crystals.
The acetomethoxy derivate of calcein (calcein AM) is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term marking of cells. The acetomethoxy group obscures the part of the molecule that chelates calcium. After transport into the cell the enzymes cut off the group, the molecule binds to calcium within cell (resulting in strong green fluorescence), and gets trapped inside. As dead cells lack this enzyme, only live cells are marked. [1]
Calcein is also used for marking freshly hatched fish[1] and for labeling of bones in live animals.
References
Acknowledgement and Attribution Regarding Sources of Content
Some of the initial content on this page may be incorporated in part from copyleft sources in the public domain including wikis such as Wikipedia and AskDrWiki. Drug information for patients came from the The National Library of Medicine. Infectious disease information may have come from the Centers for Disease Control (CDC). Differential Diagnoses are drawn from clinicians as well as an amalgamation of 3 sources: 1.The Disease Database; 2. Kahan, Scott, Smith, Ellen G. In A Page: Signs and Symptoms. Malden, Massachusetts: Blackwell Publishing, 2004:3; 3. Sailer, Christian, Wasner, Susanne. Differential Diagnosis Pocket. Hermosa Beach, CA: Borm Bruckmeir Publishing LLC, 2002:7 .
