Calciseptine

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Calciseptine
Molecular weight	7036

Calciseptine (CaS) is a natural neurotoxin isolated from the black mamba Dendroaspis p. polylepis venom. This toxin consists of 60 amino acids with four disulfide bonds. Calciseptine specifically blocks L-Type calcium channels, but not other voltage-dependent Ca²⁺ channels such as N-type and T-type channels.^[1]

Contents 1 Sequence 2 Isolation 3 Target 4 References

Sequence

Its amino acid sequence is: RICYI HKASL PRATK TCVEN TCYKM FIRTQ REYIS REGCG CPTAM WPYQT ECCKG DRCNK

Isolation

Calciseptine is purified from the venom of black mamba Dendroaspis p. polyepsis via three isolation steps: (1) gel filtration, (2) ion exchange on TSK SP 5PW, and (3) reverse-phase chromatograghy on RP18. It represents 2.8% of the total venom components. ^[1]

Target

Calciseptine blocks L-type Ca²⁺ channels.^[1] As a result, it blocks spontaneous contractions of rat portal vein, contractions of the rat thoracic aorta, uterus and cardiac preparations.

References