Cycling probe technology

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Cycling probe technology (CPT) is a molecular biological technique for detecting specfic DNA sequences. CPT operates under isothermal conditions. In some applications, CPT offers an alternative to PCR. However, unlike PCR, CPT does not generate multiple copies of the target DNA itself, and the amplification of the signal is linear, in contrast to the geometric amplification of the target DNA in PCR Tang et al. (as cited in "References") developed an integrated electrokinetic system for use in CPT.

References

• Tang, T. et al. Integrated microfluidic electrophoresis system for analysis of genetic materials using signal amplification methods. Anal. Chem. 74, 725 – 733 (2002).