Secretion assay

You don't need to be Editor-In-Chief to add or edit content to WikiDoc. You can begin to add to or edit text on this WikiDoc page by clicking on the edit button at the top of this page. Next enter or edit the information that you would like to appear here. Once you are done editing, scroll down and click the Save page button at the bottom of the page.

Secretion assay is a process used in cell biology to identify cells that are secreting a particular protein (usually a cytokine). It was first developed by Manz et al in 1995.

Usually, a cell that is secreting the protein of interest is isolated using an antibody-antibody complex that coats the cell and is able to "catch" the secreted molecules. The cell is then detected by another fluorochrome-labelled antibody, and is subsequently extracted using a process called fluorescent-activated cell sorting (FACS). The FACS method is broadly similar to the enzyme-linked immunosorbent assay (ELISA) antibody format, except that the encapsulated cells remain intact. This is advantageous as the cells are still living after the extraction has taken place.

Further advances now mean that it is possible to extract the secreting cells using a magnetic-based separation system or using a flow cytometer.

A number of commercial applications exist for secretion assay. One such example is the Gel Microdrop (GMD) techonology, developed by One Cell Systems. One Cell asserts that GMD typically recovers a higher number of viable secreting cells than other methods, whilst ignoring any cells which are not secreting the desired protein.

External links

• One Cell Systems - Gel Microdrop (GMD) Secretion Assay techonology.
• Turcanu V, Williams NA Cell identification and isolation on the basis of cytokine secretion: a novel tool for investigating immune responses Nat Med. 2001 Mar;7(3):373-6

References

1. Manz et al. (1995) Proceedings of the National Academy of Sciences USA

v • d • e Proteins: key methods of study
Experimental	Protein purification - Green fluorescent protein - Western blot - Protein immunostaining - Protein sequencing - Gel electrophoresis/Protein electrophoresis - Protein immunoprecipitation - Peptide mass fingerprinting
Bioinformatics	Protein structure prediction - Protein-protein docking - Protein structural alignment - Protein ontology - Protein-protein interaction prediction
Assay	Enzyme assay - Protein assay - Secretion assay

WikiDoc Help Menu Quick Start.. Get Started Here! How to Login How to Search Pages How to Start a New Page How to Edit a Page Cheat Sheet Editing basics Learning Basic Formatting How to make Links in WikiDoc & to External Sites How to Make Lists How to Ignoring Formatting Adding References or Footnotes to Articles Tracking Changes You Have Made How to Put an Article Into a Category When Multiple Topics Should Go To The Same Page (MI, Acute MI, AMI) Using Redirection Advanced editing How to Make Tables How to Insert Images and Other Media How to Add Video Help:How to Make Columns Web Colors How To Make Templates How to Move Pages Inserting Dynamic Dates and Times Inserting WikiDoc Statistics Editing the Table of Contents Communicating your edits Let others know what you changed: The importance of Edit Summaries Minor Edits Edit Conflicts Help Videos You Can Watch Getting Started Video

Acknowledgement and Attribution Regarding Sources of Content

Some of the initial content on this page may be incorporated in part from copyleft sources in the public domain including wikis such as Wikipedia and AskDrWiki. Drug information for patients came from the The National Library of Medicine. Infectious disease information may have come from the Centers for Disease Control (CDC). Differential Diagnoses are drawn from clinicians as well as an amalgamation of 3 sources: 1.The Disease Database; 2. Kahan, Scott, Smith, Ellen G. In A Page: Signs and Symptoms. Malden, Massachusetts: Blackwell Publishing, 2004:3; 3. Sailer, Christian, Wasner, Susanne. Differential Diagnosis Pocket. Hermosa Beach, CA: Borm Bruckmeir Publishing LLC, 2002:7 .