Fast protein liquid chromatography

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Template:Infobox chemical analysis Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography. It is very commonly used in biochemistry and enzymology. Columns used with an FPLC can separate macromolecules based on size, charge distribution, hydrophobicity, or biorecognition (as with affinity chromatography).


FPLC is a trademark of GE Healthcare, formerly Amersham-Pharmacia Biotech and Pharmacia Biotech.

Typical columns

Typical columns used for protein purification include:

See also

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